Quality British Bioinks for your research

3Dynamic Systems is now the name in high quality precision 3D Bioprinters, with our patented direct-extrusion Omega technology. It has been our objective to bring high resolution bioprinting within the reach of all tissue engineering researchers. Our 3DS Nanocellulose Bioink is the culmination of over a decade in research and optimisation, through which we have harnessed and developed a truly superior product.


20mL of 3DS Nanocellulose Bioink and crosslinking agent is only £80.00.


3Dynamic Systems is working with researchers from across the world, including the NHS to develop bioprinting technology as a means to instigate future treatments used as part of future regenerative medicine and reconstructive surgery treatments. 3DS Nanocellulose is the first completely universal bioink for 3D Bioprinting of human tissue structures. 3DS Nanocellulose Bioink is a sterile, ready-to-use product that provides an optimal cell culturing environment. The first starting kit for your 3D bioprinting needs.

Overview

The 3DS Nanocellulose Bioink kit has been specifically developed and optimised for bioprinting 3D structures. The 3DS Nanocellulose Bioink kit includes optimally formulated hydrogel complex contained in a sealed vessel for easy loading. The kit also includes 3DS Crosslinking agent, which reinforces mechanical stability.


3DS Nanocellulose Bioink - 10mL

3DS Crosslinking Agent - 5mL


The preparation procedure results in a mechanically stable 3D bioprinting structures that can be maintained for up to four-weeks in an incubator. Further to this, although the 3DS Nanocellulose Bioink was developed for purposes of 3D bioprinting, however, the kit can also be used for many other studies that require 3D culture and structure formation, including - cell biology, biochemistry, pharmacology, physiology, biomaterials sciences, bioengineering, and drug testing and tissue development.

Storage & Handling

3DS Nanocellulose Bioink complex can be stored at ambient room temperature. However, 2-8°C is recommended for a long-term storage of up to six-months. Do not freeze the bioink as this will result in reducing the quality of the structure. 3DS Crosslinking Agent can be stored at ambient room temperature. However, 2-8°C is recommended for a long-term storage of up to twelve-months.

Sterility

All Handling should be done in sterile condition to avoid contamination. 3DS Nanocellulose Bioink is sterilised by membrane filtration during the fabrication at our factory.

Instructions for Use

1. Warm the 3DS Nanocellulose Bioink sample to 28°C for approximately 30 minutes, in which it will turn into a low viscosity liquid.


2. To add cells, the mix ratio is 1:10 (1mL of 3DS Nanocellulose Bioink to 100µL of cell suspension in media). Add the two materials together and gently mix until you achieve a homogenous suspension.


3. Transfer the bioink to a suitable syringe for dispensing in a 3D Bioprinter.


4. After the bioprinting process has taken place, cover the structure with 3DS Crosslinking Agent for approximately five minutes and remove with a syringe.


5. Place the crosslinked structure into a petri dish and cover it with the appropriate growth media.


6. Add the structure to an incubator at 38°C and change the growth media every two days.


7. Any remaining bioink should be sealed and stored at 2-8°C for further use.

Note

1. Gelation time and gel stiffness can be adjusted by varying the concentration of 3DS Nanocellulose Bioink complex or 3DS Crosslinking agent. Please contact 3Dynamic Systems for additional specifications or for the fabrication of custom formulations.


2. Each kit component has been manufactured under aseptic conditions and tested for bacteria and fungus. Please employ aseptic practices to maintain the sterility of 3DS Nanocellulose Bioink throughout the preparation and 3D bioprinting operation.

Caution

Our product is for research use only. It has not been approved for use in diagnostic or therapeutic procedures.

3D Bioprinting

During the bioprinting process, the rheological properties of the 3DS Nanocellulose Bioink are important as it behaves differently depending on temperature. This needs to be maintained at 22.5°C which is approximately 0.8°C higher that the gelation temperature of 3DS Nanocellulose Bioink. Our bioink maintains the correct environment and pH to sustain cells during the 3D bioprinting process.


The toolpath of the 3D structure generated and the 3D-bioprinted ear structure post crosslinking. Direct dual extrusion 3DS Bioprinting technology developed by 3DS enables the deposition of combined hydrogel scaffolds and automated addition of crosslinking agent. A typical layer resolution of between 150-250µm is possible when working with our bioink formulation.


The software system is used to control the bioprinter and instructs each of the high precision stepper motors to deposit the materials in layers. These layers act as an independent support to the 3D structure.

Incubation

Once the 3D bioprinting process is completed, the construct is carefully removed and placed into an incubator at 38°C. During a period of up to 21-days in an incubator then micro¬-environmental parameters can be controlled. In order to produce biochemical stimuli, transforming growth factor-β1 can be added to the structure in order to effectively produce cartilage and collagen matrix. Tissue structure after three weeks of maturation.

Tissue Culture

Staining has been performed on a cross section to of our bioink to identify the presence of type VI collagen which correlated with the existence of extracellular matrix (ECM). As shown below is the formation of the final high resolution structure.


(a) Hematoxylin and eosin staining of immature cartilage tissue and auricular constructs showing condensation of cells present. (b) Immunolabeling collagen VI (shown in green) in the pericellular region of chondrocytes via nuclear staining (shown in blue).


Staining with anti-type VI collagen antigen provided significant fluorescence within the section providing evidence of the presence of the pericellular protein as shown in Figure a. By carrying out immunofluorescence with II-II6B3-s type II collagen this provided evidence for the formation chondrogenic cells, which had produce ECM as shown in Figure b. This evidence shows that cartilage structures can be formed when using 3DS Nanocellulose Bioink.

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